Optimization of the Operational Conditions to Produce Extracellular and Cell-Bound Biosurfactants by Aneurinibacillus aneurinilyticus Using Corn Steep Liquor as a Unique Source of Nutrients

The relevance of this work lies in the fact that it is first time corn steep liquor (CSL) has been proposed as a unique source nutrients for producing biosurfactants controlled fermentation and Aneurinibacillus aneurinilyticus, isolated from CSL, evaluated extracellular cell-bound fermentation, using secondary raw materials nutrients. In present study, A. aneurinilyticus was inoculated into culture medium containing sterilized CSL solutions (100–400 g L−1) incubated different temperatures (20–60 °C) times (8–30 days). dependent variables under study were concentration biosurfactant production terms critical micellar (CMC), well C/N ratio extracts. It observed could increase produced by if these fermented during 19 days at 40 °C, 250 L−1 CSL; mean value 6 favorable CMC concentrations enabled detection extracts conditions. Hence, conditions be considered optimal both CSL.